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Molecular Biology Tools

NEBcutter

This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once.

Reverse Complement

 Reverse Complement converts a DNA sequence into its reverse, complement, or reverse-complement counterpart.

BLAST

A BLAST search allows you to compare a sequence of interest with a database of sequences for homology.  It can be used to align different types of sequences.

Primer-BLAST

Finding primers specific to your PCR template

Ensembl

Information about genes, transcripts, sequences, splicing isoforms, exon sequences, gene coordinates etc.

ENCODE project

Encyclopedia of DNA Elements. Experimental data of among other things RNA-protein binding using eCLIP and RNA-seq data.

Geuvadis

This collection contains RNA-seq data generated by the Geuvadis consortium. The data includes mRNA and small RNA sequence data for over 460 samples from the 1000 Genomes project, including samples from five of the 1000 Genomes populations (CEU, FIN, GBR, TSI and YRI).

dbSNP

dbSNP is a database containng human single nucleotide variations, microsatellites, and small-scale insertions and deletions along with publication, population frequency, molecular consequence, and genomic and RefSeq mapping information for both common variations and clinical mutations.

Fruitfly

Splice site prediction tool.

Last Updated 29.10.2024