Research interest
Our research aim is to gain an understanding of the post-translational regulation of epithelial sodium transport under physiological and pathophysiological conditions. The experimental approaches are a combination of electrophysiological, imaging and molecular techniques carried out primarily on human tissue samples and cell cultures.
Methods and Techniques
- Immunohistochemistry (light-, laser scanning confocal-, multi-photon microscopy),
- Cloning
- Cell culture (Transient and stable expression systems, knock-down, CRISPR)
- Western blotting
- Quantitative PCR
- Studies on human kidney tissue
- Various transgenic and cell-specific knockout models
- Metabolic studies of renal function in animals
- Developmental changes in renal morphology and function
- Recombinant protein expression and purification
- Antibody development and characterization
- Electrophysiology: Patch Clamp (Whole-cell patch configuration, single channel recordings).
Electrophysiology: Ussing chamber studies
Staff
Lab technician Mohamed A. Ahmed, Post.doc. Morten B. Engelund, Ph.d.-student. Rikke Zachar
Courses
- Bachelor module 5 for Medical students – Electrophysiology of the heart
- SU502 for science student – General physiology and muscle cell physiology
- SU503 for science student – General physiology and muscle cell physiology
- SU503 for science students - Methods and measurements in pharmacology and Mechanisms of drug actions
- SU516 for farmaceutical students – General biology and Mechanisms of drugs actions (Per Svenningsen)
Selected publications
Svenningsen P, Andersen H, Nielsen LH, Jensen BL. Urinary serine proteases and activation of ENaC in kidney--implications for physiological renal salt handling and hypertensive disorders with albuminuria. Pflugers Arch. 2015 Mar;467(3):531-42. doi: 10.1007/s00424-014-1661-5. Epub 2014 Dec 9. PubMed PMID: 25482671.
Zachar RM, Skjødt K, Marcussen N, Walter S, Toft A, Nielsen MR, Jensen BL, Svenningsen P. The epithelial sodium channel γ-subunit is processed proteolytically in human kidney. J Am Soc Nephrol. 2015 Jan;26(1):95-106. PubMed PMID: 25060057
Svenningsen P, Nielsen MR, Marcussen N, Walter S, Jensen BL. Acta Physiol (Oxf). 2014 Oct;212(2):166-74. doi: 10.1111/apha.12323. Epub 2014 Jun 26. PMID: 24913262
Svenningsen P, Bistrup C, Friis UG, Bertog M, Haerteis S, Krueger B, Stubbe J, Jensen ON, Thiesson HC, Uhrenholt TR, Jespersen B, Jensen BL, Korbmacher C, Skøtt O.
J Am Soc Nephrol. 2009 Feb;20(2):299-310. doi: 10.1681/ASN.2008040364. Epub 2008 Dec 10. PMID: 19073825
